type III site-specific deoxyribonuclease EC#: 3.1.21.5; 凯望编码 (ChemWhat Code): 1379737

英文名 type III site-specific deoxyribonuclease
Example Structure Example Structure of type III site-specific deoxyribonuclease EC#: 3.1.21.5
别名 ATP-dependent type III restriction endonuclease, BceSI, BsaHI, cj0031, EC 3.1.23, EC 3.1.24, EcoP1, EcoP15, EcoP15I, EcoPI, HinFIII, LlaFI, MmyCI, More, NgoAXP, PhaBI, PspGI, PstII, R.EcoP15I, R.MmyCI, REase, restriction endonuclease PstII, restriction-modification system, StyLTI, type III DNA restriction/modification enzyme, type III R-M enzyme, type III R/M enzyme, type III RE, type III restriction endonuclease, type III restriction enzyme, type III restriction-modification enzyme, type III restriction-modification system, type III RM system, type III testriction-modification enzyme, type III-like restriction endonuclease
EC Number 3.1.21.5
CAS编号 9075/8/5
Comments This is a large group of enzymes which, together with those now listed as EC?3.1.21.3?(type I site-specific deoxyribonuclease) and EC?3.1.21.4?(type II site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC?3.1.23?and EC?3.1.24. They have an absolute requirement for ATP but do not hydrolyse it;?S-adenosy-L-methionine stimulates the reaction, but is not absolutely required. They recognize specific, short DNA sequences and cleave a short distance away from the recognition sequence. These enzymes exist as complexes with enzymes of similar specificity listed under EC?2.1.1.72?[site-specific DNA-methyltransferase (adenine-specific)] or EC?2.1.1.73?[site-specific DNA-methyltransferase (cytosine-specific)]. A complete listing of all of these enzymes has been produced by R.J. Roberts and is available at http://rebase.neb.com/rebase/rebase.htm.
Cofactor
History
Reactions Endonucleolytic cleavage of Dna to give specific double-stranded fragments with terminal 5′-phosphates.

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